Clinical Correlation of Dengue Strains on the Basis of Seroprevalence in a Tertiary Care Hospital.: Clinical Correlation of Dengue Strains

Ali  Haider; Faheem  Ullah; Muhammad  Bilal; Zeeshan  Saif; Hina Awais; Talha Mannan; Hasnain  Javed; Fareeha  Bashir

doi:10.54393/pbmj.v5i4.341

Authors

Ali Haider University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Faheem Ullah University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Muhammad Bilal University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Zeeshan Saif University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Hina Awais The University of lahore
Talha Mannan Department of Medical Lab technology, Shalamar School of Allied Health Sciences, Lahore, Pakistan
Hasnain Javed Provincial Public Health Reference Lab, Punjab AIDS Control Program, Lahore, Pakistan
Fareeha Bashir University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i4.341

Keywords:

Dengue, Reverse transcriptase polymerase chain reaction, Enzyme linked immunosorbent assay, White blood cells and Platelets

Abstract

Dengue viruses are icosahedral in structure and contain a single-stranded positive- sense RNA sequence of 11kb inside their capsid protein, which belongs to Flaviviridae family, genera Flavivirus. DENV a vector dependent viral virus which presents a severe health danger worldwide. Objective: To study the different strains of dengue on the basis of serotypes Methods: A cross sectional study was conducted at a tertiary care hospital Lahore, Primary and Secondary Health Department Lahore. The blood samples of 103 patients were collected from non-random sampling technique to check out the data of different parameters such as WBC’s & Platelets through Complete Blood Count (CBC), NS1 from ELISA and nature of dengue strains through RT- PCR. Results: Of 103 positive dengue patients, there were n= 58 (33.63 ± 16.54) males and n=45 (40.64± 16.00) females. Data for the total patients is subjected to statistical differences by Paired t-test (*p<0.05). The overall percentage of dengue strains within the sample population was DENV-2 (96%), DENV-3 (2%), DENV-1 & DENV-2 (2% in females, nil in males) DENV-2 & DENV-3 (2% both in males and females). According to clinical parameters the correlation of ELISA results with WBC’s was significant (<0.0001) as well as ELISA and platelets of patients had no correlation with each other. Conclusions: Leukopenia and Thrombocytopenia is found particularly in DENV-2 strain as well as we found two different strains in two patients. So, our research work is helpful for the identification in genetic similarity of dengue strains.

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