In Vitro study of Thrombolytic activity from the different parts of Carica papaya plant on COVID-19 patients.: Thrombolytic activity  of Carica papaya in COVID-19 patients

Ayesha  Afzal; Aasia  Zahid; Asma  Akbar; Zunaira  Jamal; Huma  Habib; Hina Awais; Shehla  Javaid

doi:10.54393/pbmj.v4i2.107

Authors

Ayesha Afzal University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan.
Aasia Zahid University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Asma Akbar University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan.
Zunaira Jamal University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Huma Habib University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan.
Hina Awais University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
Shehla Javaid University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v4i2.107

Abstract

Carica papaya plant has been used for medicinal purpose throughout the world because it have anti-inflammatory, anti-oxidant, antiviral, anti-cancer and wound healing like properties. Objective: To evaluate the thrombolytic activity of Carica papaya plant in normal healthy individuals and COVID-19 patients to determine either parts of the plant (roots, seeds and leaves) exhibit more activity in normal persons or in COVID-19 patients. Methods: For this study, total 20 blood samples were taken, 10 for normal individuals and 10 for positive COVID-19 patients. We used two different solvents i.e. autoclaved distilled water and concentrated methanol to prepare the 10% root, seed and leaf extracts of Carica papaya plant. For the thrombolytic activity of these plant extracts, samples were arranged as a triplicate for the accuracy of results percentage. Results: Our results evaluated that in normal individuals, distilled water root extract and methanolic seed extract exhibits maximum thrombolytic activity. The mean value with distilled water (root extract) and methanol (seed extract) is 36.9% and 32.9% respectively. While in covid-19 patients, distilled water leaf extract and methanolic leaf extract reveal maximum thrombolytic activity. In patients, the mean value with distilled water and methanol (leaf extract) is 21.8% and 23% respectively. Conclusions: In our study, we have observed that normal persons showed highly significant results as compared to COVID-19 patients. Because in COVID-19 disease, plasminogen activator inhibitor 1 (PAI-1), thrombin activatable fibrinolysis inhibitor and tissue plasminogen activator (tPA) factors elevate which results in hypo-fibrinolysis.

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