Isolation and Characterization of Antibiotic producing Lysobacter: Isolation and Characterization of Lysobacter

Nimra Cheema; Asma Waheed Qureshi

doi:10.54393/pbmj.v7i03.1059

Authors

Nimra Cheema Department of Zoology, Government College Women University, Sialkot, Pakistan
Asma Waheed Qureshi Department of Zoology, Government College Women University, Sialkot, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v7i03.1059

Keywords:

Lysobacter, Antibiotics, Rhizosphere Soil, Reasoner’s 2A agar, Pathogens,, Antibacterial

Abstract

Lysobacter species, known for their cosmopolitan distribution across diverse habitats, are promising sources of antibiotics and bioactive compounds. They showcase lytic activity against a wide range of microorganisms including human pathogens. Objective: To isolate and characterize the antibiotic producing Lysobacter bacteria. Methods: A total of 51 rhizosphere soil samples were collected from district Sialkot. The duration of this study was 7 months from April to October 2022. Out of these samples 18 antibiotics producing Lysobacter bacteria were isolated. These isolates were characterized morphologically and biochemically by standard methods. Antibiotic activity of Lysobacter was evaluated against gram negative and positive pathogenic bacteria. Four pathogens i.e., E. coli, S. aureus, S. typhi and P. vulgaris were used in this study to evaluate antibiotic activity of Lysobacter. Results: The most sensitive pathogen towards antibiotics produced by Lysobacter isolates was E. coli while the P. vulgaris showed some resistance. All antibiotics producing Lysobacter isolates were gram negative and rod shaped. The colonies of isolates were circular, mucoid and color ranges from cream white to pale yellow. All strains were catalase and oxidase positive except S14 that was oxidase negative. Conclusions: The results of this study revealed that the antibiotics producing Lysobacter isolate are effective inhibitors for both gram negative and gram positive human pathogens.

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