Production and Evaluation of Polyclonal Antibodies Against Surface Protein of Mycobacterium tuberculosis: Diagnostic and Vaccine Implications: Polyclonal Antibodies Against Surface Protein of Mycobacterium tuberculosis

Amna Bibi; Mateen Ur Rehman; Sheheryar Ahmad Khan; Muhammad Abu Baker; Barira Amir Ghauri; Muhammad Fakhar Ghaffar

doi:10.54393/pbmj.v8i10.1315

Authors

Amna Bibi Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Mateen Ur Rehman Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Sheheryar Ahmad Khan Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Muhammad Abu Baker Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Barira Amir Ghauri Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Muhammad Fakhar Ghaffar Department of Health Promotion and Public Health, Ulster University, Birmingham, United Kingdom

DOI:

https://doi.org/10.54393/pbmj.v8i10.1315

Abstract

Mycobacterium tuberculosis (MTB), which causes tuberculosis (TB), is a significant health threat in the world. The diagnosis of early active and latent TB is still a problem. Serological methods have potential benefits of offering a fast and cost-effective diagnosis, although they need to be further refined to become clinically reliable. Objectives: To generate and test polyclonal antibodies (pAbs) of surface proteins of MTB as a potentially useful immunodiagnostic application. Methods: Extraction of surface proteins of MTB was done with the use of PBS and Tween-20, and rabbits were immunized with antigen-adjuvant mixtures. The serum was gathered, and titers of antibodies were determined by an indirect ELISA. Results: Rabbits vaccinated against the surface proteins of MTB generated high-titer antibodies, and the median absorbance of the 1:1600 dilution was 1.42 0.15, which was significantly higher (p<0.001) when compared to their controls that were not immunized. The endpoint titer, which was 1:12,800, was geometric, indicating that immunization and antibody were successful. Conclusions: The paper shows that it is possible to produce polyclonal antibodies against the proteins of the surface of Mycobacterium tuberculosis, which is going to serve as a basis for the future development of serological diagnostic instruments.

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