Detection of Metalo-Beta-Lactamase Gene in Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Lahore, Pakistan: Detection of Metalo-Beta-Lactamase Gene in Pseudomonas Aeruginosa

Humera Kausar; Shabbir Hussain; Afia Muhammad Akram

doi:10.52229/pbmj.v3i1.4

Authors

Humera Kausar Department of Biotechnology, Kinnaird College for Women University, Lahore, Pakistan
Shabbir Hussain Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan
Afia Muhammad Akram Department of Zoology, Division of Science and Technology, University of Education, Lahore, Pakistan

DOI:

https://doi.org/10.52229/pbmj.v3i1.4

Abstract

Pseudomonas aeruginosa is a widespread organism, caused severe nosocomial infection in human and associated with multiple drug resistance (MDR)Objective:The present study was carried out to observe current antimicrobial resistant pattern of Pseudomonas aeruginosa in Lahore and to detect the Metallo-beta-lactamase (MBL) gene in carbapenem resistantPseudomonas aeruginosaMethods:By screening 360 samples total 123 Pseudomonas aeruginosa was identified by standard microbiology techniques such as microscopy and biochemical testing. The isolated Pseudomonas aeruginosa was evaluated for drug resistance by disc diffusion method and polymerase chain reaction(PCR) was used to identify the carbapenem resistance causing gene (bla-VIM and bla-IMP) Results:Following antibiotic resistant pattern was observed,Gentamycin (59.00%), Ceftazidime(58.7%), Ceftriaxone (58.00%), Cefotazime (57.0%) and Ciprofloxacin (55.00%). Resistance rates to carbapenem group of antibiotics is Doripenem (30.5%) Meropenem(31.0%) and Imipenem (28.0%). Out of 123 samples of Pseudomonas aeruginosa, 28 isolates were found resistant to carbapenem group of antibiotic which was supposed to be highly sensitive for this bacterium. Molecular based identification of resistance genes showed that bla-IMP gene was present in 32.1% (09) and bla-VIM was found positive in 17.8% (04) samples. Metallo-beta-lactamasesproducing genes (bla-VIM and bla-IMP), amongcarbapenem resistant Pseudomonas aeruginosa were detectedin 28.1% of samples. If other carbapenem resistant gene were also included this number might be higherConclusions:PCRbased test should be included in routine laboratory examination for quick detection of the resistancecausing genes.

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