Renal Toxicity Induced by Carbon Tetrachloride in Experimental Model: Renal Toxicity Induced by CCl4 in Experimental Model

Mirza Fahad  Baig; Muhammad Khalil  Ahmad Khan

doi:10.54393/pbmj.v6i3.897

Authors

Mirza Fahad Baig Department of Zoology, University of Okara, Renalla Khurd, Pakistan
Muhammad Khalil Ahmad Khan Department of Zoology, University of Okara, Renalla Khurd, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v6i3.897

Keywords:

CCl4, Renal Toxicity, Biochemical Parameters

Abstract

Carbon tetrachloride (CCl₄) is largely used as a solvent in chemical industries. It is also well known for hepatic and renal toxic actions. It imposes serious health threats. It is also one of the major causes that is toxic for the vital organs like lungs, kidney, liver, brain, etc. Objective: To check nephrotoxicity of Carbon Tetrachloride (CCl₄) on Rat Kidneys. Methods: The experiment was conducted at the animal house of the Department of Zoology, University of Okara. The targeted animal was Albino Rat. Two groups were designed control and experimental groups. The rats were fed with 30% diluted CCl₄ to check the toxic effect on the kidneys and normal saline to the control group for comparison. A trial for 12 days was conducted for this purpose. Sampling or dissection was done after 12 days to determine serum Urea, Creatinine, and Electrolytes Sodium (Na), and Potassium (K). Rats were dissected and the heart was punctured to take a blood sample and to collect organs. Results: We observed the increased values of Urea, Creatinine and Electrolytes, Sodium (Na), and Potassium (K) as compared to normal values, which have proved the renal toxicity was induced by CCl₄ in Albino Rats. All the experimental data were analyzed by using SPSS-19. The level of significance among the various treatments was determined by LSD at a 0.05% level of probability. Conclusions: These findings underline the substantial health risks that CCl₄ poses and emphasize the necessity of putting preventative measures and safety regulations in place.

References

Unsal V, Cicek M, Sabancilar İ. Toxicity of carbon tetrachloride, free radicals and role of antioxidants. Reviews on Environmental Health. 2021 Jun; 36(2): 279-95. doi: 10.1515/reveh-2020-0048. DOI: https://doi.org/10.1515/reveh-2020-0048

Satyanarayana PS, Singh D, Chopra K. Quercetin, a bioflavonoid, protects against oxidative stress-related renal dysfunction by cyclosporine in rats. Methods and Findings in Experimental and Clinical Pharmacology. 2001 May; 23(4): 175-81. doi: 10.1358/mf.2001.23.4.634641. DOI: https://doi.org/10.1358/mf.2001.23.4.634641

Ogeturk M, Kus I, Colakoglu N, Zararsiz I, Ilhan N, Sarsilmaz M. Caffeic acid phenethyl ester protects kidneys against carbon tetrachloride toxicity in rats. Journal of Ethnopharmacology. 2005 Feb; 97(2): 273-80. doi: 10.1016/j.jep.2004.11.019. DOI: https://doi.org/10.1016/j.jep.2004.11.019

Manna P, Sinha M, Sil PC. Aqueous extract of Terminalia arjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complementary and Alternative Medicine. 2006 Dec; 6 :1-10. doi: 10.1186/1472-6882-6-33. DOI: https://doi.org/10.1186/1472-6882-6-33

Adewole S, Salako A, Doherty O, Naicker T. Effect of melatonin on carbon tetrachloride-induced kidney injury in Wistar rats. African Journal of Biomedical Research. 2007 May; 10(2): 153-164. doi: 10.4314/ajbr.v10i2.50619. DOI: https://doi.org/10.4314/ajbr.v10i2.50619

Ganie SA, Haq E, Masood A, Zargar MA. Amelioration of carbon tetrachloride induced oxidative stress in kidney and lung tissues by ethanolic rhizome extract of Podophyllum hexandrum in Wistar rats. Journal of Medicinal Plants Research. 2010 Aug; 4(16): 1673-7.

Teschke R. Liver injury by carbon tetrachloride intoxication in 16 patients treated with forced ventilation to accelerate toxin removal via the lungs: A clinical report. Toxics. 2018 Apr; 6(2): 25. doi: 10.3390/toxics6020025. DOI: https://doi.org/10.3390/toxics6020025

Faroon O. Toxicological profile for carbon tetrachloride. Agency for Toxic Substances and Disease Registry; 2005.

Chapman K, Prabhudesai M, Erdman Jr JW. Effects of ethanol and carbon tetrachloride upon vitamin A status of rats. Alcoholism: Clinical and Experimental Research. 1992 Aug; 16(4): 764-8. doi: 10.1111/j.1530-0277.1992.tb00675.x. DOI: https://doi.org/10.1111/j.1530-0277.1992.tb00675.x

Adams EM, Spencer HC, Rowe VK, McCollister DD, Irish DD. Vapor toxicity of carbon tetrachloride determined by experiments on laboratory animals. AMA Archives of Industrial Hygiene and Occupational Medicine. 1952; 6(1): 50-66.

Khan MR, Rizvi W, Khan GN, Khan RA, Shaheen S. Carbon tetrachloride-induced nephrotoxicity in rats: Protective role of Digera muricata. Journal of Ethnopharmacology. 2009 Feb; 122(1): 91-9. doi: 10.1016/j.jep.2008.12.006. DOI: https://doi.org/10.1016/j.jep.2008.12.006

Khan RA, Khan MR, Sahreen S, Bokhari J. Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food and Chemical Toxicology. 2010 Aug; 48(8-9): 2469-76. doi: 10.1016/j.fct.2010.06.016. DOI: https://doi.org/10.1016/j.fct.2010.06.016

Lin HM, Tseng HC, Wang CJ, Lin JJ, Lo CW, Chou FP. Hepatoprotective effects of Solanum nigrum Linn extract against CCl4-iduced oxidative damage in rats. Chemico-Biological Interactions. 2008 Feb; 171(3): 283-93. doi: 10.1016/j.cbi.2007.08.008. DOI: https://doi.org/10.1016/j.cbi.2007.08.008

Srinivasan M, Rukkumani R, Ram Sudheer A, Menon VP. Ferulic acid, a natural protector against carbon tetrachloride‐induced toxicity. Fundamental & Clinical Pharmacology. 2005 Aug; 19(4): 491-6. doi: 10.1111/j.1472-8206.2005.00332.x. DOI: https://doi.org/10.1111/j.1472-8206.2005.00332.x

Anjaneyulu M, Tirkey N, Chopra K. Attenuation of cyclosporine-induced renal dysfunction by catechin: possible antioxidant mechanism. Renal Failure. 2003 Jan; 25(5): 691-707. doi: 10.1081/JDI-120024285. DOI: https://doi.org/10.1081/JDI-120024285

Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K. Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology. 2003 Aug; 62(2): 353-6. doi: 10.1016/S0090-4295(03)00255-3. DOI: https://doi.org/10.1016/S0090-4295(03)00255-3

Bartels H and Bohmer M. Microestimation of creatinine. Clinica Chimica Acta. 1971; 32: 81-5. doi: 10.1016/0009-8981(71)90467-0. DOI: https://doi.org/10.1016/0009-8981(71)90467-0

Tabacco A, Meiattini F, Moda E, Tarli P. Simplified enzymic/colorimetric serum urea nitrogen determination. Clinical Chemistry. 1979 Feb; 25(2): 336-7. doi: 10.1093/clinchem/25.2.0336a. DOI: https://doi.org/10.1093/clinchem/25.2.0336a

Simerville JA, Maxted WC, Pahira JJ. Urinalysis: a comprehensive review. American Family Physician. 2005 Mar; 71(6): 1153-62.

Bhattacharya H and Lun L. Biochemical effects to toxicity of CCl 4 on rosy barbs (Puntius conchonius). Our Nature. 1970 Jan; 3(1): 20-5. doi: 10.3126/on.v3i1.330. DOI: https://doi.org/10.3126/on.v3i1.330

Bhadauria M, Nirala SK, Shukla S. Multiple treatment of propolis extract ameliorates carbon tetrachloride induced liver injury in rats. Food and Chemical Toxicology. 2008 Aug; 46(8): 2703-12. doi: 10.1016/j.fct.2008.04.025. DOI: https://doi.org/10.1016/j.fct.2008.04.025

Xu JY, Su YY, Cheng JS, Li SX, Liu R, Li WX, et al. Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity and nephrotoxicity in rats. Carbon. 2010 Apr; 48(5): 1388-96. doi: 10.1016/j.carbon.2009.12.029. DOI: https://doi.org/10.1016/j.carbon.2009.12.029

Eden E, Harrison DD. Studies in Carbon Tetrachloride Poisoning. Australian Journal of Experimental Biology & Medical Science. 1955 Feb; 33(1): 85-89. doi: 10.1038/icb.1955.9. DOI: https://doi.org/10.1038/icb.1955.9

Huxtable RJ. Physiological actions of taurine. Physiological Reviews. 1992 Jan; 72(1): 101-63. doi: 10.1152/physrev.1992.72.1.101. DOI: https://doi.org/10.1152/physrev.1992.72.1.101

Vengal Rao P, Ashok Kumar CK, Ashwini G, Ambareesh Kumar R, Sangeetha S. Evaluation of Hepatoprotective Activity of Santallum Album (Stem) Against Paracetamol Induced Hepatotoxicity in Albino Wistar Rats. International Journal of Innovative Pharmaceutical Research. 2014; 5(1): 370-373.