Phenotypic Detection Of Antibiotic Resistance And Production Of Extended Spectrum Beta lactamases In E.COLI Isolated From UTI Patients At (HMC): Phenotypic detection of antibiotic resistance and beta-lactamases in e. coli isolated from UTI

Umair  AKbar; Muhammad  Ali; Fawad Ali; Yamin  Rashid; Ikramullah .; Khan Niaz  Khan

doi:10.54393/pbmj.v5i4.360

Authors

Umair AKbar Institute of Biotechnology & Microbiology Bacha Khan University Charsadda, Kp Pakistan
Muhammad Ali Institute of Biotechnology and Microbiology Bacha Khan University Charsadda, KPK Pakistan
Fawad Ali Institute of Biotechnology and Microbiology Bacha Khan University Charsadda, KPK Pakistan
Yamin Rashid SR Medicine & Allied, Swat Medical College, Swat, KPK, Pakistan
Ikramullah . Department of Agriculture, Bacha Khan University, Charsadda, KPK, Pakistan
Khan Niaz Khan Department of Biology, Edwardes College Peshawar, KPK, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i4.360

Keywords:

antibiotic resistance, E. coli, beta-lactamases, UTI patients

Abstract

This study aimed at the isolation of antibiotic resistance and ESBL producing E. coli from urinary tract infection patients visiting HMC hospital Peshawar. Method: A total of 200 samples were collected and cultured on selective media for bacterial isolation. Two hundred isolates were subjected to different morphological and biochemical tests for the isolation and identification of E. coli. A total of 121 E. coli isolations were recovered. These E. coli isolates were subjected to antibiotic sensitivity testing. Eight antibiotics (Meropenem, Tazocin, Ciprofloxacin, Fosfomycin, Cefotaxime, Augmentin, Ceftazidime, Cefepime) were used for this purpose. Result: The isolates showed high resistance to these antibiotics such as meropenem having percent resistance of 1.85 while sensitivity is 98.14%, Tazocin showed percent resistance of 9.25 and sensitivity is 85.18%, Ciprofloxacin showed resistance of 87.96 percent, sensitivity is 12.03%, Fosfomycin showed resistance of 3.70 percent and sensitivity is 95.37%, Cefotaxime, Augmentin, Ceftazidime, Cefepime showed resistance of 100 percent. The isolates were then investigated for the existence of ESBL enzymes production through double disc diffusion test. Among the 121 isolates, 108 were positive for ESBL enzymes production. Conclusion: Current findings highlight the high prevalence of multi drug resistant and ESBL positive isolates of E. coli in clinical samples. Proper management and control measures are needed to control the spread of these highly pathogenic E. coli isolates to ensure public health safety.

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