Determination of Proximate Composition of Nigella sativa L. Seeds and its Effective Role in Improving Lipid Profile Among Hyperlipidaemic Women: Proximate composition of Nigella sativa L. seeds and role in improvement of lipid profile.

Rida  Zanib; Shahnai  Basharat; Tara  khursheed; Zarnain  Ali Shah; Zainab  Gulzar; Muhammad  Shaheer; Sakina  Fatima

doi:10.54393/pbmj.v5i7.626

Authors

Rida Zanib University Institute of Diet and Nutritional sciences. The university of Lahore, Lahore, Pakistan
Shahnai Basharat University Institute of Diet and Nutritional sciences. The university of Lahore, Lahore, Pakistan
Tara khursheed University Institute of Diet and Nutritional sciences. The university of Lahore, Lahore, Pakistan
Zarnain Ali Shah University Institute of Diet and Nutritional sciences. The university of Lahore, Lahore, Pakistan
Zainab Gulzar University Institute of Diet and Nutritional sciences. The university of Lahore, Lahore, Pakistan
Muhammad Shaheer Department of Human Nutrition The university of Agriculture, Peshawar Pakistan.
Sakina Fatima Institute Of Food And Nutrition Science, Pir Mehar Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i7.626

Keywords:

Nigella sativa L. seed powder, Black cumin, Blood Lipid profile, Hyperlipidaemia, AOAC, Hypolipidemia, Lipid ameliorating effect, Proximate analysis

Abstract

Hyperlipidaemia is characterized by abnormally elevated levels of body’s plasma cholesterol and triacylglycerol. Objective: To find out the proximate composition of Nigella sativa L. and its hypolipidemic effect on blood lipid profile among hyperlipidaemic women. Methods: To determine the proximate composition of Nigella sativa seed the kjeldhah method, soxhlet extraction using petroleum ether, and AOAC procedure were used. For the identification of hypolipidemic impact, a total of 64 women with mild to moderate hyperlipidaemia, aged between 25-35 years were selected for the 8 weeks of study. The sample was selected from the University of Lahore Teaching Hospital, Lahore. After removing physical contaminants like dirt, dust, and other foreign grains black seeds were air dried. After drying, seeds were grounded into fine powder by using commercial blender. Each capsule was prepared with 500mg powder of N. sativa, and was transferred in air tight jars. After screening the participants of experimental group, they were advised to use 2 capsules of N. sativa supplement before breakfast and two capsules in the afternoon prior to their meal for 8 weeks. Participants were also advised to follow the given 7-day diet plan for 8 weeks. The anthropometric measurements, biochemical evaluation (lipid profile), and dietary intake data were collected. Results: The mean age group of Hyperlipidaemic patients enrolled in study was 32.5±0.34 years. There was a significant reduction in Low Density Lipoprotein (LDL), total cholesterol level, and triglyceride levels with p-value less than 0.05. High density lipoprotein levels were also improved from 49.5±8.38mg/dl to 51.81±10.21mg/dl. Conclusion: The study concluded that Nigella sativa L. seed powder supplementation showed a significant improvement in lipid profile of hyperlipidaemia patients. The study found an increase in high density lipoprotein among patients.

References

Quintana HK, Janszky I, Gigante B, Druid H, Ahlbom A, Hallqvist J, et al. Diabetes, hypertension, overweight and hyperlipidaemia and 7-day case-fatality in first myocardial infarction. IJC Metabolic & Endocrine. 2016 Sep; 12:30-5. doi: 10.1016/j.ijcme.2016.05.009.

Rao W, Su Y, Yang G, Ma Y, Liu R, Zhang S, et al. Cross-Sectional Associations between Body Mass Index and Hyperlipidaemia among Adults in Northeastern China. International Journal of Environmental Research and Public Health. 2016 May; 13(5):516. doi: 10.3390/ijerph13050516.

Shah J, Alsubaie AS, Sehar B, Khan FS, Omer EO, Almoajel AM, et al. Association of Cigarette Smoking with Hyperlipidaemia in Male Individuals. Food and Nutrition Sciences. 2021 Oct; 12(10):937-49. doi: 10.4236/fns.2021.1210069

Regmi M, Rehman A. Familial Hyperlipidaemia Type. Stat-Pearls [Internet]. Treasure Island (FL): StatPearls Publishing. 2019 Dec.

Liu YT, Nfor ON, Wang L, Hsu SY, Lung CC, Tantoh DM, et al. Interaction between Sex and LDLR rs688 Polymorphism on Hyperlipidaemia among Taiwan Biobank Adult Participants. Biomolecules. 2020 Feb; 10(2):244. doi: 10.3390/biom10020244.

Ford I, Murray H, McCowan C, Packard CJ. Long-Term Safety and Efficacy of Lowering Low-Density Lipoprotein Cholesterol with Statin Therapy: 20-Year Follow-Up of West of Scotland Coronary Prevention Study. Circulation. 2016 Mar; 133(11):1073-80. doi: 10.1161/Circulationaha.115.019014.

Mollazadeh H, Hosseinzadeh H. The protective effect of Nigella sativa against liver injury: a review. Iranian Journal of Basic Medical Sciences. 2014 Dec; 17(12):958-66.

Ziaee T, Moharreri N, Hosseinzadeh H. Review of pharmacological and toxicological effects of Nigella sativa and its active constituents. Journal of Medicinal Plants. 2012 May; 11(42):16-42.

Boskabady MH, Vahedi N, Amery S, Khakzad MR. The effect of Nigella sativa alone, and in combination with dexamethasone, on tracheal muscle responsiveness and lung inflammation in sulfur mustard exposed guinea pigs. Journal of Ethnopharmacology. 2011 Sep; 137(2):1028-34. doi: 10.1016/j.jep.2011.07.030.

Ramadan MF. Nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa L.): an overview. International journal of food science & technology. 2007 Oct; 42(10):1208-18. Doi: 10.1111/j.1365-2621.2006.01417.x.

Ghosheh OA, Houdi AA, Crooks PA. High performance liquid chromatographic analysis of the pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L.). Journal of Pharmaceutical and Biomedical Analysis. 1999 Apr; 19(5):757-62. doi: 10.1016/s0731-7085(98)00300-8

Ibrahim RM, Hamdan NS, Mahmud R, Imam MU, Saini SM, Rashid SN, et al. A randomised controlled trial on hypolipidemic effects of Nigella Sativa seeds powder in menopausal women. Journal of Translational Medicine. 2014 Mar; 12:82. doi: 10.1186/1479-5876-12-82.

Burkhardt R. Hyperlipidaemia and cardiovascular disease: new insights on lipoprotein(a). Journal of Current Opinions in Lipidology. 2019 Jun; 30(3):260-261. doi: 10.1097/MOL.0000000000000594.

Zaid M, Hasnain S. Plasma lipid abnormalities in Pakistani population: trends, associated factors, and clinical implications. Brazilian Journal of Medical and Biological Research. 2018 Jul; 51(9):e7239. doi: 10.1590/1414-431X20187239.

Razmpoosh E, Safi S, Nadjarzadeh A, Fallahzadeh H, Abdollahi N, Mazaheri M, et al. The effect of Nigella sativa supplementation on cardiovascular risk factors in obese and overweight women: a crossover, double-blind, placebo-controlled randomized clinical trial. European Journal of Nutrition. 2021 Jun; 60(4):1863-1874. doi: 10.1007/s00394-020-02374-2.

Farzaneh E, Nia FR, Mehrtash M, Mirmoeini FS, Jalilvand M. The Effects of 8-week Nigella sativa Supplementation and Aerobic Training on Lipid Profile and VO2 max in Sedentary Overweight Females. International Journal of Preventive Medicine. 2014 Feb; 5(2):210-6.

Farhangi MA, Dehghan P, Tajmiri S. Powdered black cumin seeds strongly improves serum lipids, atherogenic index of plasma and modulates anthropometric features in patients with Hashimoto's thyroiditis. Lipids in Health and Disease. 2018 Mar; 17(1):59. doi: 10.1186/s12944-018-0704-x.

Khoddami A, Ghazali HM, Yassoralipour A, Ramakrishnan Y, Ganjloo A. Physicochemical characteristics of nigella seed (Nigella sativa L.) oil as affected by different extraction methods. Journal of the American Oil Chemists' Society. 2011 Apr; 88(4): 533-40.

Farzaneh E, Nia FR, Mehrtash M, Mirmoeini FS, Jalilvand M. The Effects of 8-week Nigella sativa Supplementation and Aerobic Training on Lipid Profile and VO2 max in Sedentary Overweight Females. International Journal of Preventive Medicine. 2014 Feb; 5(2):210-6.

Farhangi MA, Dehghan P, Tajmiri S, Abbasi MM. The effects of Nigella sativa on thyroid function, serum Vascular Endothelial Growth Factor (VEGF) - 1, Nesfatin-1 and anthropometric features in patients with Hashimoto's thyroiditis: a randomized controlled trial. BMC complementary and alternative medicine. 2016 Nov; 16(1):471. doi: 10.1186/s12906-016-1432-2.