Serological Distribution and Hematological Profiling of Dengue Virus Infection at Hyderabad and Adjoining Areas: A Cross-Sectional Study: Serological Distribution and Hematology Analysis of Dengue Virus Infection

Raja Rehan; Beenish Khanzada; Saba Naz; Zaib Un Nisa Mughal; Ghulam Rasool Rind

doi:10.54393/pbmj.v9i3.1356

Authors

Raja Rehan Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
Beenish Khanzada Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
Saba Naz Institute of Chemistry, University of Sindh, Jamshoro, Pakistan
Zaib Un Nisa Mughal Department of Physiology, University of Sindh, Jamshoro, Pakistan
Ghulam Rasool Rind Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v9i3.1356

Keywords:

Serology, Dengue NS1, Fever, Myalgia, Mortality

Abstract

Pakistan appears to be a dengue endemic area with seasonal outbreaks and a high spike every year. Objectives: To investigate the period prevalence (positivity rate) of dengue viral infection (DVI) among suspected cases in the hospital and other factors related to DVI in Hyderabad district, Sindh, Pakistan, between August and December 2025. Methods: A cross-sectional study to find out the period prevalence of DVI through a hospital survey and specially designed Questionnaires including 20-60 years’ age patients complaining of dengue-specific symptoms. Dengue NS1 antigen test, IgM, IgG, and CBC were performed, and results were interpreted using the chi-square test via SPSS version 22.0. Results: Out of 544 patients, 255 cases were dengue positive. 244 (95%) were found to be dengue NS1 antigen positive, 8 (3%) were IgG positive, and 3 (1%) were positive with IgM. Age and gender revealed no significant statistical association with DVI. However, more males (49%) in the age range of 20-30 years were majorly affected by the dengue virus. Seasonal variation study depicted October as the month badly hit by DVI with a major surge in Dengue, i.e., 140 positive cases. The Hyderabad main city area and Qasimabad were majorly affected by DVI. The most common symptoms observed in dengue-positive cases included muscle and joint pain, vomiting, headache, and nausea accompanied by fever. Conclusions: The research finds that dengue infection is in a significant upsurge in Hyderabad, and the majority of the positive cases are in the early acute stage (NS1-positive).

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