Comparison of p16 with human papillomavirus with oral squamous cell carcinoma and oral premalignant lesion: Human Papillomavirus with Oral Squamous Cell Carcinoma

Mohammad  Manzoor; Muhammad Zaib  Khan; Adnan  Sarwar; Fatima  Daud; Inam-u llah; Hina Mir; Bushra  Tahreem; Muhammad  Awais; Sudhair Abbas  Bangash; Fares M  Muthanna

doi:10.54393/pbmj.v4i2.230

Authors

Mohammad Manzoor Pathology Department, Bacha Khan Medical College Mardan
Muhammad Zaib Khan Dental Surgeon Consultant, Endodontics, Safari Hospital, Rawalpindi
Adnan Sarwar Department of Pathology, Pak International Medical College, Peshawar
Fatima Daud Department of Anatomy, Pak International Medical College, Peshawar
Inam-u llah Department of Food Science, The University of Haripur, KPK, Pakistan
Hina Mir Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar
Bushra Tahreem Department of Life Sciences, School of Science, University of Management and Technology, Lahore Pakistan
Muhammad Awais Department of Life Sciences, School of Science, University of Management and Technology, Lahore Pakistan
Sudhair Abbas Bangash Faculty of Life Science, Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar
Fares M Muthanna Department of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand

DOI:

https://doi.org/10.54393/pbmj.v4i2.230

Keywords:

P16; Oral Premalignant Lesions; Oral Squamous Cell Carcinoa; Human Papillomavirus (16/18)

Abstract

Worldwide Oral carcinomas considerable problem. It is crucial to know the molecular incidences of molecular carcinogenesis, as it has a significant association with tobacco in Pakistani population. So, this study aimed to screen out the savage high risk of papilloma virus 16/18 and p16 in oral premalignant lesion and oral squamous cell carcinoma. Moreover, the p16 correlation between HPV and OPL and OSCC was also analyzed. Method: A total of 150 samples from the oral cavity were taken from the Hayatabad Medical complex (50 samples of OPL and 100 samples of OSCC).In this study we used immunohistochemistry to look for p16 in OPL and OSCC, and we used polymerase chain reaction to find HPV. SPSS 21 was used to input and evaluate the data. The relationship between HPVandp16withdifferentvariables was determined using Chi-square and Fisher exact tests. Results:For p16, 14% of 50 oral premalignant lesions were found positive and 86% were negative. Moreover, while screening for the OSCC, the 18 percent individuals were found positive for OSCC, while the remaining (82%) screened out negative. HPV was diagnosed in 6% of 50 OPL. The co-occurrence of HPV with p16 was found in all the 15 (100%) individual’s with a p value of 0.001. However, three of the 18 cases with p16 expression did not have HPV infection. Conclusion: The current research supports the use of p16 as a unique marker for human papilloma virus in oral squamous cell carcinomas. Furthermore, a chemical carcinogen like cigarettes is thought to be one of the main risk factors for p16 and HPV infection, as well as other things.

References

Ali, S. A., Awan, M. S., Atif, S., Ali, N., Mirza, Y. J. T. J. o. l., & otology. (2018). Correlation of human papillomavirus infection and clinical parameters with five-year survival in oral squamous cell carcinoma. 132(7), 628-635.

https://doi.org/10.1017/S0022215118000361

Baig, S., Zaman, U., & Lucky, M. H. J. I. J. o. I. D. (2018). Human papilloma virus 16/18: Fabricator of trouble in oral squamous cell carcinoma. 69, 115-119.

https://doi.org/10.1016/j.ijid.2018.02.003

Jeon, J.-H., Kim, M. G., Park, J. Y., Lee, J. H., Kim, M. J., Myoung, H., . . . surgery, r. (2017). Analysis of the outcome of young age tongue squamous cell carcinoma. 39(1), 1-7.

https://doi.org/10.1186/s40902-017-0139-8

Jiang, X., Wu, J., Wang, J., & Huang, R. J. T. i. d. (2019). Tobacco and oral squamous cell carcinoma: A review of carcinogenic pathways. 17.

https://doi.org/10.18332/tid/105844

Klein, S., Quaas, A., Quantius, J., Löser, H., Meinel, J., Peifer, M., . . . von Knebel Doeberitz, M. J. C. C. R. (2021). Deep Learning Predicts HPV Association in Oropharyngeal Squamous Cell Carcinomas and Identifies Patients with a Favorable Prognosis Using Regular H&E Stains. 27(4), 1131-1138.

https://doi.org/10.1158/1078-0432.CCR-20-3596

Lang Kuhs, K. A., Kreimer, A. R., Trivedi, S., Holzinger, D., Pawlita, M., Pfeiffer, R. M., . . . Waterboer, T. J. C. (2017). Human papillomavirus 16 E 6 antibodies are sensitive for human papillomavirus-driven oropharyngeal cancer and are associated with recurrence. 123(22), 4382-4390.

https://doi.org/10.1002/cncr.30966

Mascitti, M., Tempesta, A., Togni, L., Capodiferro, S., Troiano, G., Rubini, C., . . . Limongelli, L. J. O. d. (2020). Histological features and survival in young patients with HPV‐negative oral squamous cell carcinoma. 26(8), 1640-1648.

https://doi.org/10.1111/odi.13479

Meng, H.-x., Miao, S.-s., Chen, K., Li, H.-n., Yao, G., Geng, J., . . . Mao, X. J. B. r. i. (2018). Association of p16 as prognostic factors for oropharyngeal cancer: evaluation of p16 in 1470 patients for a 16 year study in Northeast China. 2018.

https://doi.org/10.1155/2018/9594568

Mills, A. M., Dirks, D. C., Poulter, M. D., Mills, S. E., & Stoler, M. H. J. T. A. j. o. s. p. (2017). HR-HPV E6/E7 mRNA in situ hybridization. 41(5), 607-615.

https://doi.org/10.1097/PAS.0000000000000800

Molony, P., Kharytaniuk, N., Boyle, S., Woods, R. S., O'Leary, G., Werner, R., . . . neck. (2017). Impact of positive margins on outcomes of oropharyngeal squamous cell carcinoma according to p16 status. 39(8), 1680-1688.

https://doi.org/10.1002/hed.24824

Naqvi, S. U., Khan, S., Ahmed, A., Lail, A., Gul, S., & Ahmed, S. J. J. o. M. V. (2020). Prevalence of EBV, CMV, and HPV in oral squamous cell carcinoma patients in the Pakistani population. 92(12), 3880-3883.

https://doi.org/10.1002/jmv.25791

Oliveira, L. R., Ribeiro-Silva, A., Costa, J. P. O., Simões, A. L., Di Matteo, M. A. S., Zucoloto, S. J. O. S., Oral Medicine, Oral Pathology, Oral Radiology,, & Endodontology. (2008). Prognostic factors and survival analysis in a sample of oral squamous cell carcinoma patients. 106(5), 685-695.

https://doi.org/10.1016/j.tripleo.2008.07.002

Pandey, P., Ralli, M., Dixit, A., Agarwal, S., Chaturvedi, V., Sawhney, A., . . . Pathology, M. (2021). Assessment of immunohistochemical expression of p16 in head and neck squamous cell carcinoma and their correlation with clinicopathological parameters. 25(1), 74.

https://doi.org/10.4103/jomfp.JOMFP_252_20

Pastrez, P. R. A., Mariano, V. S., da Costa, A. M., Silva, E. M., Scapulatempo-Neto, C., Guimarães, D. P., . . . Sichero, L. J. J. o. C. (2017). The relation of HPV infection and expression of p53 and p16 proteins in esophageal squamous cells carcinoma. 8(6), 1062.

https://doi.org/10.7150/jca.17080

Perera, M., Al-Hebshi, N., Perera, I., Ipe, D., Ulett, G., Speicher, D., . . . Johnson, N. J. J. o. d. r. (2018). Inflammatory bacteriome and oral squamous cell carcinoma. 97(6), 725-732.

https://doi.org/10.1177/0022034518767118

Rathish, D., Wijerathne, B., Khan, R. J. I. J. o. O., Head, & Surgery, N. (2020). Human Papillomavirus-Associated Oral Squamous Cell Carcinoma Among Adults Living in South-Central Asia: A Systematic Review. 1-6.

https://doi.org/10.1007/s12070-020-01842-9

Samim, D., Méan, M., Clair, C., & Marques-Vidal, P. J. P. o. (2018). A 10-year observational study on the trends and determinants of smoking status. 13(7), e0200010.

https://doi.org/10.1371/journal.pone.0200010

Thomson, P., Goodson, M., Smith, D. J. J. o. O. P., & Medicine. (2017). Profiling cancer risk in oral potentially malignant disorders-A patient cohort study. 46(10), 888-895.

https://doi.org/10.1111/jop.12625

Thomson, P. J. J. o. O. P., & Medicine. (2018). Perspectives on oral squamous cell carcinoma prevention-proliferation, position, progression and prediction. 47(9), 803-807.

https://doi.org/10.1111/jop.12733