Comparison of the Antibacterial Activities of Different Antibiotics Against Clinical Isolates: Antibiotic Resistance Patterns

Ansar  Abbas; Ammarah  Hasnain; Sarosh Malik; Maria Farid  Siddiqui; Samia  Chaudhry

doi:10.54393/pbmj.v5i1.250

Authors

Ansar Abbas Virtual University,Lahore, Pakistan
Ammarah Hasnain Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Sarosh Malik University Institute of Radiological Sciences and Imaging Technology, Faculty of Allied Health Sciences, The University of Lahore, Lahore, Pakistan
Maria Farid Siddiqui Department of Pharmacy, The University of Lahore, Lahore, Pakistan
Samia Chaudhry Services institute of Medical Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i1.250

Keywords:

sensitivity, resistance, antibiotics, evolution, Bacterial isolates

Abstract

Antibiotic resistance is the capacity of certain strains of bacteria to develop a tolerance to specific antibiotics to which they were once sensitive Objective: To evaluate the antibiotic resistance by measuring the zone of inhibition in terms of sensitivity S, and resistance R. Methods: A total of 1000 clinical isolates collected from different samples were obtained. Antibacterial activities were evaluated by performing antibiotic susceptibility pattern of all clinical isolates against 20 commercial antibiotic discs (Oxicillin, Gatifloxacin, Ampicillin, Levofloxacin, Meteronidazole, Ofloxacin, Tazocin, Cefotaxime, Ciprofloxacin, Augmintin, Vancomycin, Linezolid, Fusidic Acid, Nalidixic Acid, Erythromycin, Klaricid, Amoxicillin, Gentamycin, Norfloxacin And Vibramycin) by using Kirby-Bauer disc diffusion method. Results: A total of 1000 clinical isolates had been identified where Staphylococcus Aureus showed the highest prevalence 410(41.0%), next to Pseudomonas species 144(14.4%), Escherichia coli 131(13.1%), Enterobacter 64(6.4%) and Klebsiella species 31(3.1%). Vancomycin and Tazocin showed highest sensitivity percentage (77.9%) and (75.1%) for most of the clinical isolates, next to Meteronidazole (71.2%), Klaricid (58.0%), and Fusidic Acid (52.0%), Vibramycin (42.0%), Ciprofloxacin (38.4%), and Ofloxacin (35.7%). Conclusions: It is needed to maintain the appropriate antibiotic usage, prescription by evaluating the antibiotic susceptibility pattern and proper hand washing recommended.

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