Antibacterial effect of Tradescantia pallida purpurea against fish (Labeo rohita) pathogens

Antibacterial effects of Tradescantia pallida purpurea against fish pathogens


  • Syeda Shazia Bokhari Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Roheela Yasmeen Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Rimsha Manzoor Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Uzma Rafi Department of Biology, Lahore Garrison University, Lahore, Pakistan
  • Aisha Waheed Qureshi Department of Biology, Lahore Garrison University, Lahore, Pakistan



Diseases are recognized as one of the major restrictions to sustainable animal production which can cause significant economic loss in aquaculture. Even though there are various chemotherapeutic agents such as antibiotics and disinfectants in practice from centuries. Antibiotics have the potential to cause unwanted side effects as well as microbes get resistant against them. Now there is also a lot of research going to see the potential of natural products against pathogens. Tradescantia pallida purpurea plant has various bioactive compounds such as alkaloids, flavonoids, tannis and phenolic compounds Objective:The present study was conducted to testefficacy of Tradescantia pallida purpurea in chloroform extract against fish pathogens and also a comparison in methods (well diffusion and disc diffusion) was made Methods:Four fish pathogens (NP1, NP4, SCC4 and TS1) were isolated after morphological and biochemical tests. Erythromycin was used as control. 10 µg/ml chloroform leaf extract was used by well diffusion and disc diffusion Results:It was noticed that well-diffusion assay was better to inhibit growth of fish isolates as compared to disc diffusion method. It was noticed that Tradescantia pallidahaspotential to inhibit growth of pathogens. Conclusions:So, there is a need to plan a comprehensive study to recommend the maximum dose that is helpful to control fish pathogens.


Quesada, SP, Paschoal, JAR, & Reyes, FGR (2013). Considerations on the aquaculture development and on the use of veterinary drugs: special issue for fluoroquinolones—a review. Journal of food science, 78(9), R1321-R1333.

Rico, A, Phu, TM, Satapornvanit, K, Min, J, Shahabuddin, AM, Henriksson, PJ, & Van den Brink, PJ (2013). Use of veterinary medicines, feed additives and probiotics in four major internationally traded aquaculture species farmed in Asia. Aquaculture, 412, 231-243.

Romero, J, Feijoó, CG, & Navarrete, P (2012). Antibiotics in aquaculture–use, abuse and alternatives. Health and environment in aquaculture, 159.

Cabello, FC, (2006). Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol.8, 1137–1144.

Pasnik, DJ, Evans, JJ, Panangala, VS, Klesius, PH, Shelby, RA,& Shoemaker, CA (2005). Antigenicity of Streptococcus agalactiae extracellular products and vaccine efficacy. Journal of fish diseases, 28(4), 205-212.

Sakai, M. (1999). Current research status of fish immunostimulants. Aquaculture, 172(1-2), 63-92.

Harikrishnan, R, Balasundaram, C, & Heo, MS (2011). Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317(1-4), 1-15.

Al-Saif, SSAL, Abdel-Raouf, N., El-Wazanani, HA, & Aref, IA (2014). Antibacterial substances from marine algae isolated from Jeddah coast of Red sea, Saudi Arabia. Saudi Journal of Biological Sciences, 21(1), 57-64.

Alghazeer, R, Whida, F, Abduelrhman, E, Gammoudi, F, & Naili, M (2013). In vitro antibacterial activity of alkaloid extracts from green, red and brown macroalgae

Madhuri, S, Mandloi, AK, Govind, P, & Sahni, YP (2012). Antimicrobial activity of some medicinal plants against fish pathogens.

Alsaid, M, Daud, H, Bejo, SK, & Abuseliana, A (2010). Antimicrobial activities of some culinary spice extracts against Streptococcus agalactiae and its prophylactic uses to prevent streptococcal infection in red hybrid tilapia (Oreochromis sp.). World Journal of Fish and Marine Sciences,2(6), 532-538.

Baskaran, SA, Kazmer, GW, Hinckley, L, Andrew, SM, & Venkitanarayanan, K (2009). Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogens in vitro. Journal of Dairy Science, 92(4), 1423-1429.

Lee, S, Najiah, M, Wendy, W, & Nadirah, M (2009). Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites. Frontiers of Agriculture in China, 3(3), 332-336.

Dubber, D, & Harder, T (2008). Extracts of Ceramium rubrum, Mastocarpus stellatus and Laminaria digitata inhibit growth of marine and fish pathogenic bacteria at ecologically realistic concentrations. Aquaculture, 274(2-4), 196-200.

Makkar, H. P. S., Francis, G., & Becker, K. (2007). Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. Animal, 1(9), 1371-1391.

Mendes, M, Pereira, R, Pinto, IS, Carvalho, AP, & Gomes, AM (2013). Antimicrobial activity and lipid profile of seaweed extracts from the North Portuguese Coast. International Food Research Journal, 20(6), 3337.

Syahidah, A, Saad, CR, Daud, HM, & Abdelhadi, YM (2015). Status and potential of herbal applications in aquaculture: A review. Iranian Journal of Fisheries Sciences,14(1), 27-44.

Forwood, JM, Harris, JO, & Deveney, MR (2013). Efficacy of bath and orally administered praziquantel and fenbendazole against L epidotrema bidyana M urray, a monogenean parasite of silver perch, B idyanus bidyanus (Mitchell). Journal of Fish Diseases, 36(11), 939-947.

Citarasu, T (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquaculture International, 18(3), 403-414.

Jing P, Bomser JA, Schwartz SJ, He J, Magnuson BA, et al. (2008) Structurefunction relationships of anthocyanins from various anthocyaninrich extracts on the inhibition of colon cancer cell growth. J Agric Food Chem56(20): 9391-9398.

Blumenthal, M, Goldberg, A, & Brinckmann, J (2000). Herbal Medicine-Integrative Medicine Communications. Austin, 8, 401.

Logambal, SM, Venkatalakshmi, S, & Michael, RD (2000). Immunostimulatory effect of leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters). Hydrobiologia, 430(1-3), 113-120.

Panigrahi, A, Azad, IS, (2007). Microbial intervention for better fish health in aquaculture: the Indian scenario. Fish Physiol. Biochem.33, 429–440.

Parasa, LS, Tumati, SR, Prasad, CS, & Kumar, LCA (2012). In vitro antibacterial activity of culinary spices aniseed, star anise and cinnamon against bacterial pathogens of fish. International Journal of Pharmaceutical Science,4, 667-670.

Zilberg, D, Tal, A, Froyman, N, Abutbul, S, Dudai, N, & Golan‐Goldhirsh, A (2010). Dried leaves of Rosmarinus officinalis as a treatment for streptococcosis in tilapia. Journal of Fish Diseases, 33(4), 361-369.

Olusola, SE, Emikpe, BO, & Olaifa, FE (2013). The potentials of medicinal plant extracts as bio-antimicrobials in aquaculture. Int. J. Med. Aromat. Plants, Zheng, Z. L.,from western coast of Libya. African Journal of Biotechnology, 12(51), 7086-7091.

Santos APM, Segura Muñoz SI, Nadal M, Schuhmacher M, Domingo JL, et al.(2015) Traffic-related air pollution biomonitoring with Tradescantia pallida (rose) Hunt. cv. purpurea Boom in Brazil. Environ Monit Assess187(2): 39.

Li, CY, Chen, SC, Li, CY, Gau, M., & Huang, CM (2011). Randomised controlled trial of the effectiveness of using foot reflexology to improve quality of sleep amongst Taiwanese postpartum women. Midwifery, 27(2), 181-186.

Sun L, Zhang J, Lu X, Zhang L, Zhang Y (2011) Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L) leaves. Food Chem Toxicol49(10): 2689-2696.

Tona, L, Kambu, K, Ngimbi, N, Cimanga, K, & Vlietinck, AJ (1998). Antiamoebic and phytochemical screening of some Congolese medicinal plants. Journal of Ethnopharmacology, 61(1), 57-65.

Rusenova, N, & Parvanov, P (2009). Antimicrobial activities of twelve essential oils against microorganisms of veterinary importance. Trakia Journal of Sciences, 7(1).

Tan, JBL, Yap, WJ, Tan, SY, Lim, YY, & Lee, SM (2014). Antioxidant content, antioxidant activity, and antibacterial activity of five plants from the commelinaceae family. Antioxidants, 3(4), 758-769.

Huq, S(2015). Comparative phytochemical evaluation and biological activity screening of Murdannia nudiflora and Tradescantia pallida.



DOI: 10.52229/pbmj.v1i2.44
Published: 2018-12-31

How to Cite

Bokhari, S. S. ., Yasmeen, R. ., Manzoor, . R. ., Rafi, U. ., & Qureshi, A. W. . (2018). Antibacterial effect of Tradescantia pallida purpurea against fish (Labeo rohita) pathogens: Antibacterial effects of Tradescantia pallida purpurea against fish pathogens. Pakistan BioMedical Journal, 1(2).



Original Article